mouse il-17 quantikine elisa kit  (Bio-Techne corporation)

Journal: Acta Pharmaceutica Sinica. B

Article Title: Antisense oligonucleotides targeting IRF4 alleviate psoriasis

doi: 10.1016/j.apsb.2025.05.004

Figure Lengend Snippet: IRF4 deficiency alleviates the severity of IMQ-induced psoriasis. (A) Scoring curves for back skin thickness, scaling, and erythema. (B) H&E staining of skin sections. For (A, B), n = 4–5/group, results from one independent experiment. (C) RNA-seq results for whole skin showing the differential expression of genes associated with cytokines, chemokines, AMPs, epidermis development, tissue repair, and phagocytosis between IMQ-treated Irf4 +/+ and Irf4 −/− mice ( n = 3 biologically independent samples). (D) qPCR results for pathogenic factors in skin from IMQ-treated mice ( n = 7). (E) Correlation between IL-17A and IRF4 mRNA expression in peripheral blood cells from patients with psoriasis ( n = 20) and healthy controls ( n = 21). (F) ELISA results for IL-17 in mouse serum ( n = 5). (G) IL-17A immunostaining of mouse skin sections ( n = 3). Scale bar = 200 μm. (H) Immunofluorescent microscopy images of mouse skin sections stained with anti-CD4 (green), anti-IL-17A (pink), and DAPI (blue). The dashed rectangular box shows CD4 + IL-17A + cells in the dermis. Left: scale bar = 100 μm; Right: scale bar = 20 μm. (I) Flow data statistics for various types of cells. F and I, n = 4–5/group, results from 2 independent experiments. Data are presented as mean ± SD. For (A), two-sided unpaired Student’s t -test; for (D, F, I), two-sided Mann–Whitney test and two-sided unpaired Student’s t- test; for (E), linear regression analysis. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; n.s, no significance.

Article Snippet: IL-17 levels in mouse serum and splenic and human cell culture supernatants were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits (#M1700 and #D1700; R&D Systems, Minneapolis, MN, USA).

Techniques: Staining, RNA Sequencing, Quantitative Proteomics, Expressing, Enzyme-linked Immunosorbent Assay, Immunostaining, Microscopy, MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: Antisense oligonucleotides targeting IRF4 alleviate psoriasis

doi: 10.1016/j.apsb.2025.05.004

Figure Lengend Snippet: IRF4 inhibits IL-17A production in CD4 + T cells through the EP300, ROR γ t, and IL-17A pathways. (A) qPCR results for IRF4 , EP300 , and IL-17A mRNA expression as indicated ( n = 3). (B) ELISA results for IL-17 in CD4 + T cell supernatants as indicated ( n = 3). (C) Western blots of IRF4 and IL-17A in CD4 + T cells treated with CD3/CD28, ASO15, and an IRF4 overexpression plasmid. (D) Western blots of IRF4, EP300, ROR γ t, and IL-17A in MACS-sorted peripheral CD4 + T cells from patients with psoriasis. (E) ELISA results for IL-17 in CD4 + T cell supernatants as indicated in (D) ( n = 3). (F) Western blots of IRF4, EP300, ROR γ t, and IL-17A in CD4 + T cells treated with CD3/CD28, ASO15, and SGC-CBP30. (G) qPCR results for IRF4 , EP300 , RORC , IL-17A , and inflammatory-related gene expression in MACS-sorted peripheral CD4 + T cells from patients with psoriasis ( n = 3). (H) ELISA results for IL-17 in CD4 + T cell supernatants ( n = 3). (I) Western blots of IRF4, EP300, ROR γ t, and IL-17A in CD4 + T cells treated with CD3/CD28, ASO2, and SGC-CBP30. (J) qPCR results for IRF4, EP300 , RORC , IL-17A , and inflammatory-related gene expression in MACS-sorted peripheral CD4 + T cells from patients with psoriasis ( n = 3). (K) ELISA results for IL-17 in CD4 + T cell supernatants ( n = 3). Data are presented as mean ± SD. For (A, B, E), one-way analysis of variance; for (G, J), two-sided Mann–Whitney test; for (H, K), two-sided unpaired Student’s t -test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: IL-17 levels in mouse serum and splenic and human cell culture supernatants were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits (#M1700 and #D1700; R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Over Expression, Plasmid Preparation, Gene Expression, MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: Antisense oligonucleotides targeting IRF4 alleviate psoriasis

doi: 10.1016/j.apsb.2025.05.004

Figure Lengend Snippet: Targeted inhibition of IRF4 inhibits IMQ-induced psoriasis-like symptoms. (A) Representative images of mouse back skin and spleens. (B) Scoring curves for back skin thickness, scaling, and erythema. (C) H&E staining of skin sections. Scale bar = 200 μm. (D) Representative images of IL-17A-stained mouse skin sections ( n = 3). Scale bar = 200 μm. (E) Representative flow cytometry plots and statistics for CD45 + , CD3 + , CD4 + , γδ T + , IL-17A + CD45 + , IL-17A + CD3 + , and IL-17A + CD4 + . (F) qPCR results for Irf4 , Ep300 , Rorc , Il17a , and inflammatory gene expression in skin from the ASO–NC and ASO15 groups. (G) ELISA results for IL-17 levels in serum derived from the ASO–NC and ASO15 groups. (H) Western blots of IRF4, EP300, ROR γ t, and IL-17A in the skin from the ASO–NC and ASO15 groups. For (A–G), n = 3–4/group from one independent experiment. Data are presented as mean ± SD. For (B), two-sided Mann–Whitney test; for (E, G), two-sided unpaired Student’s t -test; for (F), two-sided Mann–Whitney test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001; n.s, no significance.

Article Snippet: IL-17 levels in mouse serum and splenic and human cell culture supernatants were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits (#M1700 and #D1700; R&D Systems, Minneapolis, MN, USA).

Techniques: Inhibition, Staining, Flow Cytometry, Gene Expression, Enzyme-linked Immunosorbent Assay, Derivative Assay, Western Blot, MANN-WHITNEY